OMIP‐115: High‐Dimensional Phenotypic Characterization of Human Natural Killer Cells for Therapeutic Use

• Published in: Cytometry. Part A Vol. 107; no. 6; pp. 372 - 377
• Main Authors: Christensen, Emil Birch, Schaefer, Moritz, Barnkob, Mike Bogetofte, Nielsen, Christian, Barington, Torben
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.06.2025; Wiley Subscription Services, Inc
• Subjects: Biological activity; Biomarkers; Blood; Cell Differentiation; Cell therapy; Cytotoxicity; Differentiation (biology); Flow cytometry; Flow Cytometry - methods; Humans; immunophenotyping; Immunophenotyping - methods; Killer Cells, Natural - cytology; Killer Cells, Natural - immunology; Leukocytes (mononuclear); Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - immunology; Natural killer cells; NK cells; PBMCs; Peripheral blood mononuclear cells; Phenotype; spectral flow cytometry; NK cells; immunophenotyping; PBMCs; spectral flow cytometry
• ISSN: 1552-4922; 1552-4930; 1552-4930
• DOI: 10.1002/cyto.a.24941

ABSTRACT This 29‐color flow cytometry panel was developed and optimized for in‐depth characterization of human peripheral blood NK cells for preclinical development and monitoring of NK cell therapies. The panel includes markers associated with NK cell differentiation, cytotoxicity, tissue residency, as well as NK cell dysfunction. Panel optimization was performed on freshly isolated and ex vivo activated NK cells enriched from human peripheral blood mononuclear cells (PBMCs). Overall, this panel functions as a tool to extensively characterize human NK cells, paving the way for rapid and standardized approaches to evaluate the biological activity of therapeutic NK cell products.