Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats

• Published in: Developmental neurobiology (Hoboken, N.J.) Vol. 71; no. 7; pp. 650 - 661
• Main Authors: Gross, Johann, Stute, Kerstin, Fuchs, Julia, Angerstein, Maximilian, Amarjargal, Nyamaa, Mazurek, Birgit
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.07.2011
• Subjects: Animals; Animals, Newborn; Anion Transport Proteins - biosynthesis; Anion Transport Proteins - genetics; Base Sequence; Binding Sites; Butyric acid; Butyric Acid - metabolism; Butyric Acid - pharmacology; Cell culture; Data processing; Deacetylation; GATA-3 protein; GATA3 Transcription Factor - biosynthesis; GATA3 Transcription Factor - genetics; Gata‐3; Gene Expression; Gene Expression Regulation - genetics; Hearing; Histone deacetylase; Molecular Sequence Data; Neonates; Nervous system; Nuclear receptors; Organ Culture Techniques; Organ of Corti; Organ of Corti - drug effects; Organ of Corti - metabolism; outer hair cells; Polymerase chain reaction; prestin; Promoter Regions, Genetic - genetics; Rats; Retinoic acid; Retinoids; Reverse Transcriptase Polymerase Chain Reaction; Sodium butyrate; Statistical analysis; Transcription factors; Tretinoin - metabolism; Tretinoin - pharmacology
• ISSN: 1932-8451; 1932-846X; 1932-846X
• DOI: 10.1002/dneu.20881

Prestin is the motor protein of the outer hair cells of the organ of Corti and a key factor in ensuring a high level of sensitivity of mammalian hearing. The factors that influence prestin expression are still largely unknown. We studied the effects of the application of retinoic acid, a ligand of a nuclear receptor, and of butyric acid, an inhibitor of histone deacetylase activity, on the expression of mRNA of prestin and Gata‐3 in the organotypic culture of the organ of Corti of newborn rats using RT‐PCR. Application of retinoic acid at concentrations of 1–50 μM results in a dose‐dependent expression decrease after two days in culture. Treatment with sodium butyrate (0.5–2 mM) elevated the expression of prestin and Gata‐3. Statistically significant correlations between Gata‐3 and prestin mRNA levels were observed under all conditions. The data indicate that retinoid nuclear transcription factors, GATA‐3 and histone acetylation/deacetylation processes may have a regulatory role to play in prestin expression. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 71: 650–661, 2011