Surface chemistries for antibody microarrays

Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain...

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Bibliographic Details
Published inFrontiers in bioscience Vol. 12; no. 8-12; pp. 3956 - 3964
Main Authors Seurynck-Servoss, Shannon L, Baird, Cheryl L, Rodland, Karin D, Zangar, Richard C
Format Journal Article
LanguageEnglish
Published United States 01.05.2007
Subjects
Antibodies - immunology
Enzyme-Linked Immunosorbent Assay
Surface Properties
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Summary:Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.
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ISSN:1093-9946
1093-4715
DOI:10.2741/2362