Integration-Free Reprogramming of Lamina Propria Progenitor Cells

Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinic...

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Bibliographic Details
Published inJournal of dental research Vol. 95; no. 8; pp. 882 - 888
Main Authors Howard-Jones, R.A., Cheung, O.K.Y., Glen, A., Allen, N.D., Stephens, P.
Format Journal Article
LanguageEnglish
Published Los Angeles, CA SAGE Publications 01.07.2016
SAGE PUBLICATIONS, INC
Subjects
Amino acids
Cells, Cultured
Cellular Reprogramming - physiology
Dentistry
Deoxyribonucleic acid
DNA
Ectoderm
Endoderm
Fibroblasts
Humans
Integration
KLF4 protein
Lamina propria
Medicine
Mesoderm
Mouth Mucosa - cytology
Mouth Mucosa - physiology
Mucosa
Oct-4 protein
Penicillin
Plasmids
Plasmids - genetics
Pluripotency
Pluripotent Stem Cells - physiology
Population
Precision medicine
Progenitor cells
Reverse Transcriptase Polymerase Chain Reaction
Stem cells
Stem Cells - physiology
Transcription factors
Wound healing
human induced pluripotent stem cells
hiPSCs
stem cells
IPS cells
oral mucosa
regenerative medicine
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Summary:Producing induced pluripotent stem cells (iPSCs) from human tissue for use in personalized medicine strategies or therapeutic testing is at the forefront of medicine. Therefore, identifying a source of cells to reprogram that is easily accessible via a simple noninvasive procedure is of great clinical importance. Reprogramming these cells to iPSCs through nonintegrating methods for genetic manipulation is paramount for regenerative purposes. Here, we demonstrate reprogramming of oral mucosal lamina propria progenitor cells from patients undergoing routine dental treatment. Reprogramming was performed utilizing nonintegrating plasmids containing all 6 pluripotency genes (OCT4, SOX2, KLF4, NANOG, LIN28, and cMYC). Resulting iPSCs lacked genetic integration of the vector genes and had the ability to differentiate down mesoderm, ectoderm, and endoderm lineages, demonstrating pluripotency. In conclusion, oral mucosal lamina propria progenitor cells represent a source of cells that can be obtained with minimal invasion, as they can be taken concurrently with routine treatments. The resulting integration-free iPSCs therefore have great potential for use in personalized medicine strategies.
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ISSN:0022-0345
1544-0591
DOI:10.1177/0022034516637579