Markers of type D retroviruses in children with Burkitt's-type lymphoma

• Published in: Immunology letters Vol. 53; no. 2; pp. 101 - 104
• Main Authors: Kzhyshkowska, Julia G., Kiselev, Alexander V., Gordina, Galina A., Kurmashow, Valerij I., Portjanko, Natalia M., Ostashkin, Alexander S., Ilyin, Konstantin V.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.11.1996
• Subjects: Adolescent; AIDS/HIV; Antibodies, Viral - analysis; Antibodies, Viral - isolation & purification; Blotting, Southern; Blotting, Western; Burkitt Lymphoma - etiology; Burkitt Lymphoma - virology; Burkitt's-type lymphoma; Cells, Cultured; Child; Child, Preschool; Children's lymphoproliferative diseases; DNA, Viral - analysis; DNA, Viral - isolation & purification; Female; Hodgkin Disease - etiology; Hodgkin Disease - virology; Humans; Lymphocytes; Lymphoma, Large-Cell, Anaplastic - etiology; Lymphoma, Large-Cell, Anaplastic - virology; Lymphoma, T-Cell - etiology; Lymphoma, T-Cell - virology; Lymphoproliferative Disorders - etiology; Lymphoproliferative Disorders - virology; Male; Mason-Pfizer monkey virus; Mason-Pfizer monkey virus - genetics; Mason-Pfizer monkey virus - immunology; Mason-Pfizer monkey virus - isolation & purification; Polymerase Chain Reaction; Precursor Cell Lymphoblastic Leukemia-Lymphoma - etiology; Precursor Cell Lymphoblastic Leukemia-Lymphoma - virology; Seroepidemiologic Studies; Type D retroviruses; Burkitt's-type lymphoma; Mason-Pfizer monkey virus; Children's lymphoproliferative diseases; Type D retroviruses
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/S0165-2478(96)02625-9

Antibodies to gag-coded proteins of type D retroviruses have been detected in children with lymphadenopathy [1]. We tested 41 HIV noninfected children with lymphoproliferative diseases (27 cases of Burkitt's-type lymphoma, six cases of Hodgkin's disease, four cases of T-cell lymphoma, three cases of lymphoblastic lymphoma and one case of large-cell anaplastic lymphoma) for the presence of type D retroviral serological and genetical markers. Twenty-five healthy donors were tested as a control. DNA samples from peripheral blood lymphocytes were analyzed by the polymerase chain reaction (PCR) and Southern blotting for the presence of type D retroviral related sequences. MPMV pro-pol specific sequences have been detected in 18 out of 27 children with Burkitt's-type lymphoma. By means of Western blotting, six patients positive in PCR/Southern blotting analysis were also found to contain Mason-Pfizer monkey virus (MPMV) specific antibodies, in their sera. All children with other lymphoproliferative diseases as well as healthy donors were negative in PCR/Southern blotting and Western blotting analysis. These data suggest the possible association of type D retroviral markers with Burkitt's-type lymphoma of children.