Synthesis of transthyretin (pre-albumin) mRNA in choroid plexus epithelial cells, localized by in situ hybridization in rat brain

• Published in: The journal of histochemistry and cytochemistry Vol. 34; no. 7; pp. 949 - 952
• Main Authors: Stauder, AJ, Dickson, PW, Aldred, AR, Schreiber, G, Mendelsohn, FA, Hudson, P
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.07.1986; SAGE Publications; Histochemical Society
• Subjects: Animals; Biochemistry and metabolism; Biological and medical sciences; Brain Chemistry; Central nervous system; Choroid Plexus - metabolism; Choroid Plexus - ultrastructure; Computers; Densitometry; DNA; Epithelium - metabolism; Epithelium - ultrastructure; Fundamental and applied biological sciences. Psychology; Genetic Markers; Male; Nucleic Acid Hybridization; Prealbumin - biosynthesis; Prealbumin - genetics; Rats; Rats, Inbred Strains; RNA, Messenger - biosynthesis; Transferrin - genetics; Vertebrates: nervous system and sense organs; Choroid plexus; Transferrin; Rat; In situ; Rodentia; Central nervous system; Brain (Vertebrata); Cerebral ventricle; Hybridization; Metabolism; Autoradiography; Vertebrata; Mammalia; Messenger RNA; Carrier protein
• ISSN: 0022-1554; 1551-5044
• DOI: 10.1177/34.7.3458812

The sites of synthesis of transthyretin in the brain were investigated using in situ hybridization with [35S]-labeled recombinant cDNA probes specific for transthyretin mRNA. Autoradiography of hybridized coronal sections of rat brain revealed specific cellular localization of transthyretin mRNA in choroid plexus epithelial cells of the lateral, third, and fourth ventricles. Transferrin mRNA was also investigated and, in contrast to transthyretin mRNA, was localized mainly in the lateral ventricles. Our results indicate that substantial synthesis of transthyretin and transferrin mRNA may occur in the choroid plexus.