Batch operational stability of immobilized heterologous Rhizopus oryzae lipase during acidolysis of virgin olive oil with medium-chain fatty acids

• Published in: Biochemical engineering journal Vol. 67; pp. 265 - 268
• Main Authors: Nunes, P.A., Pires-Cabral, P., Guillén, M., Valero, F., Ferreira-Dias, S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.08.2012; Elsevier
• Subjects: Acidolysis; Biocatalysis; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Enzyme deactivation; Fundamental and applied biological sciences. Psychology; General aspects; Heterologous Rhizopus oryzae lipase; Immobilization; Immobilization techniques; Methods. Procedures. Technologies; Olea; Rhizopus oryzae; Structured lipids; Immobilization; Structured lipids; Biocatalysis; Acidolysis; Heterologous Rhizopus oryzae lipase; Enzyme deactivation; Zygomycota; Deactivation; Stability; Enzyme; Triacylglycerol lipase; Lipids; Esterases; Olive oil; Fatty acids; Carboxylic ester hydrolases; Fungi; Rhizopus oryzae; Batchwise; Hydrolases
• ISSN: 1369-703X; 1873-295X
• DOI: 10.1016/j.bej.2012.06.004

► Rhizopus oryzae heterologous lipase (r-ROL) was immobilized in different supports. ► r-ROL was able to catalyze acidolysis of olive oil with caprylic or capric acid. ► r-ROL was reused, either without or with biocatalyst rehydration between batches. ► Operational stability of r-ROL in Lewatit VPOC 1600 increased with rehydration. Structured triacylglycerols containing medium-chain fatty acids (M) at positions sn-1,3 and long-chain fatty acids (L) at the sn-2 position (MLM type), were obtained by acidolysis of virgin olive oil with caprylic or capric acid, in solvent-free media, at 40°C, catalyzed by a heterologous Rhizopus oryzae lipase (r-ROL) immobilized in Eupergit® C or in Lewatit VP OC 1600. The biocatalyst immobilized in Eupergit® was reused in consecutive 23-h batches with rehydration of the biocatalyst between batches. A first-order deactivation was observed (half-life time, t1/2=39.0h for caprylic; t1/2=54.3h for capric acid). During acidolysis with capric acid catalyzed by r-ROL immobilized in Lewatit VP OC 1600, without rehydration, a first-order deactivation was observed (t1/2=49.1h); with rehydration, a considerable increase in stability was observed (t1/2=234h; Sadana's series-type inactivation kinetics model).