Monoclonal antibodies to gonadotropin-releasing hormone (GnRH) inhibit binding of the hormone to its receptor

Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes cor...

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Published inHybridoma and hybridomics Vol. 21; no. 4; p. 281
Main Authors Gangatirkar, Pradnya, Gangadharan, Sunil, Narendranath, Anita, Nagpal, Sushma, Salunke, Dinakar M, Karande, Anjali A
Format Journal Article
LanguageEnglish
Published United States 01.08.2002
Subjects
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Cell Line
Cells, Cultured
Epitope Mapping
Epitopes - chemistry
Epitopes - genetics
Gonadotropin-Releasing Hormone - chemistry
Gonadotropin-Releasing Hormone - genetics
Gonadotropin-Releasing Hormone - immunology
Gonadotropin-Releasing Hormone - metabolism
Hybridomas - immunology
Luteinizing Hormone - secretion
Mice
Molecular Sequence Data
Neutralization Tests
Pituitary Gland - metabolism
Pituitary Gland - secretion
Proto-Oncogene Proteins c-fos - metabolism
Rats
Receptors, LHRH - metabolism
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Summary:Monoclonal antibodies (MAbs) specific to gonadotropin-releasing hormone (GnRH) were obtained using different strategies of conjugation of the peptide to carrier protein and immunization. Of several antibodies obtained, two, namely F1D3C5 and E2D2 bound GnRH in solution phase. Though the epitopes corresponding to the two overlapped, there was a one amino acid shift in the core epitope. These two antibodies were characterized with respect to inhibition of GnRH induced responses in rat pituitary cultures and alpha-T3.1 mouse gonadotrope cell line.
ISSN:1536-8599
DOI:10.1089/153685902760213895