Evidence for a spectrin-like protein as a major component of the synaptosomal membrane cytoskeleton

• Published in: Biochemical and biophysical research communications Vol. 115; no. 2; pp. 437 - 443
• Main Authors: Hesketh, J.E., Thierse, D., Aunis, D.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.09.1983
• Subjects: Animals; Cytoskeleton - analysis; Electrophoresis, Polyacrylamide Gel; Isoelectric Focusing; Male; Molecular Weight; Rats; Spectrin - analysis; Synaptic Membranes - ultrastructure

After treatment of synaptosomes with Nonidet-containing buffers, a proportion of the proteins remained insoluble. The major component (50%) of the residue was identified as a spectrin-like protein by immunodetection after mono- and bi-dimensional gel electrophoresis and transfer to nitrocellulose pa...