Evidence for a spectrin-like protein as a major component of the synaptosomal membrane cytoskeleton

After treatment of synaptosomes with Nonidet-containing buffers, a proportion of the proteins remained insoluble. The major component (50%) of the residue was identified as a spectrin-like protein by immunodetection after mono- and bi-dimensional gel electrophoresis and transfer to nitrocellulose pa...

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Published inBiochemical and biophysical research communications Vol. 115; no. 2; pp. 437 - 443
Main Authors Hesketh, J.E., Thierse, D., Aunis, D.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.09.1983
Subjects
Animals
Cytoskeleton - analysis
Electrophoresis, Polyacrylamide Gel
Isoelectric Focusing
Male
Molecular Weight
Rats
Spectrin - analysis
Synaptic Membranes - ultrastructure
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Summary:After treatment of synaptosomes with Nonidet-containing buffers, a proportion of the proteins remained insoluble. The major component (50%) of the residue was identified as a spectrin-like protein by immunodetection after mono- and bi-dimensional gel electrophoresis and transfer to nitrocellulose paper. Actin was also present.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(83)80163-6