DNA-chlorpheniramine interaction studied by spectroscopic techniques

• Published in: Biochimica et biophysica acta Vol. 1379; no. 1; pp. 129 - 133
• Main Authors: Medina, Miguel Ángel, Ramı́rez, Francisco Javier, Ruiz-Chica, Joaquı́n, Chavarrı́a, Teresa, López-Navarrete, Juan T, Sánchez-Jiménez, Francisca
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 08.01.1998
• Subjects: Chlorpheniramine; Chlorpheniramine - metabolism; Chlorpheniramine - pharmacology; Diamines - metabolism; DNA - chemistry; DNA - metabolism; DNA structure; Electrophoresis, Agar Gel; Ethidium - metabolism; Fluorometry; FTIR; Histamine H1 Antagonists - pharmacology; Intercalating Agents - metabolism; Laser Raman; Nucleic Acid Conformation; Plasmids - metabolism; Polyamine; Spectroscopy, Fourier Transform Infrared; Spectrum Analysis, Raman; DNA structure; Chlorpheniramine; Polyamine; CPA, chlorpheniramine; EtBr, ethidium bromide; Laser Raman; FTIR; CT-DNA, calf-thymus DNA; ODC, ornithine decarboxylase; Spi, spermine
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(97)00093-7

It was previously studied that the antihistaminic chlorpheniramine elicits a biphasic response on cell growth and regulates polyamine metabolism, as described for polyamines. In part, polyamine effects on macromolecular synthesis and cell growth are attributed to nucleic acid:polyamine interactions. In this work, we have tested the hypothesis of a DNA:chlorpheniramine interaction, using fluorometry, FTIR and Raman spectroscopic techniques. The results indicate that DNA:chlorpheniramine interaction occurs inducing conformational changes in the macromolecule by affecting both phosphodiester bonds and bases. Results open new perspectives for characterization of action mechanisms of natural or synthetic diamines with pharmacological or physiological importance.