Urinary 3-methoxy-4-hydroxyphenylglycol determination using reversed-phase chromatography with amperometric detection

A reversed-phase liquid chromatographic method with amperometric detection has been developed for the determination of urinary 3-methoxy-4-hydroxyphenylglycol. Before and after enzymatic deconjugation, it was purified by an extraction procedure and rapidly quantified under isocratic conditions. The...

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Bibliographic Details
Published inJournal of chromatography Vol. 233; no. 1; pp. 89 - 95
Main Authors Santagostino, Giuseppe, Frattini, Pietro, Schinelli, Sergio, Cucchi, Maria Laura, Corona, Gian Luigi
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 10.12.1982
Subjects
Adult
Chromatography, High Pressure Liquid
Chromatography, Liquid - methods
Electrochemistry
Female
Glucuronidase
Glycols - urine
Humans
Male
Methoxyhydroxyphenylglycol - isolation & purification
Methoxyhydroxyphenylglycol - urine
Multienzyme Complexes
Sulfatases
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Summary:A reversed-phase liquid chromatographic method with amperometric detection has been developed for the determination of urinary 3-methoxy-4-hydroxyphenylglycol. Before and after enzymatic deconjugation, it was purified by an extraction procedure and rapidly quantified under isocratic conditions. The 24-h excretion profile in normal human subjects (eight males and seven females) was determined; our results are consistent with those arrived at in a number of other studies. The present method is highly sensitive and selective; in addition, a good degree of precision is assured by use of 4-methoxy-3-hydroxyphenylglycol as internal standard.
ISSN:0378-4347
DOI:10.1016/S0378-4347(00)81734-X