lncRNA ENSMUST00000134285 Increases MAPK11 Activity, Regulating Aging-Related Myocardial Apoptosis

• Published in: The journals of gerontology. Series A, Biological sciences and medical sciences Vol. 73; no. 8; p. 1010
• Main Authors: Chun Yang, Xiao, Hui Zhao, Dong, Bond Lau, Wayne, Qiang Liu, Ke, Yu Tian, Jia, Chao Cheng, Zi, Liang Ma, Xin, Hua Liu, Jing, Fan, Qian
• Format: Journal Article
• Language: English
• Published: United States 09.07.2018
• Subjects: Aging - metabolism; Animals; Apoptosis; Blotting, Western; Mice; Mitogen-Activated Protein Kinase 11 - metabolism; Myocardium - metabolism; Myocytes, Cardiac - metabolism; Oligonucleotide Array Sequence Analysis; Real-Time Polymerase Chain Reaction; RNA, Long Noncoding - physiology
• ISSN: 1758-535X
• DOI: 10.1093/gerona/gly020

Previous studies have demonstrated that aging promotes myocardial apoptosis after ischemia/reperfusion, via unknown specific mechanisms. The present study investigates the potential relationship between lncRNAs and aging-related apoptosis by lncRNA/mRNA microarray technology. The results indicate aging increased myocardial lncRNA ENSMUST00000134285 and mMAPK11, confirmed by both bioinformatics analysis and polymerase chain reaction (PCR). Mouse cardiomyocytes were subjected to gene manipulation (ENSMUST00000134285 knockdown and overexpression). Knockdown of ENSMUST00000134285 inhibited MAPK11 activity and increased the myocardial apoptotic ratio (determined by TUNEL staining and caspase activity assays) after hypoxia/reoxygenation. Conversely, overexpression of ENSMUST00000134285 increased MAPK11 activity and decreased the myocardial apoptotic ratio. Furthermore, luciferase reporter assay revealed that miR760 may be a mediator between ENSMUST00000134285 and mMAPK11. We have provided evidence that lncRNAs are the important regulatory molecules in aging-mediated effects upon apoptosis. The apoptosis regulatory effects of aging are complex. Except apoptosis-promoting effects, aging could also inhibit myocardial apoptosis after hypoxia or ischemia. Further studies investigating the mechanisms that aging inhibit myocardial apoptosis after hypoxia/ischemia.