In Vivo Evaluation of the Antitumor and Immunogenic Properties of Silver and Sodium Dichloroacetate Combination against Melanoma

Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents to be used in melanoma treatment. This strategy is designed to increase the activity of these two compounds that affect DNA integrity and the mitochondria at different levels. Furthermore, we eva...

Full description

Saved in:
Bibliographic Details
Published inJournal of nanomaterials Vol. 2020; no. 2020; pp. 1 - 8
Main Authors Zamora-Ávila, Diana Elisa, Hernández-Vidal, Gustavo, Kawas-Garza, Jorge Ramsy, Rodríguez-Padilla, Cristina, Santana-Krímskaya, Silvia Elena, Franco-Molina, Moisés Armides, Torres-Cavazos, Zaida, Moreno-Degollado, Gustavo
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 2020
Hindawi
Hindawi Limited
Subjects
Anticancer properties
Apoptosis
Cancer therapies
Cell death
Cell growth
Colorimetry
Deoxyribonucleic acid
DNA
Evaluation
Experiments
Flow cytometry
Formulations
Glucose
Laboratories
Melanoma
Metabolism
Mitochondria
Nanomaterials
Nanoparticles
Nitric oxide
Penicillin
Silver
Sodium
Software
Tumors
United States > US
Mexico
Online AccessGet full text

Cover

More Information
Summary:Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents to be used in melanoma treatment. This strategy is designed to increase the activity of these two compounds that affect DNA integrity and the mitochondria at different levels. Furthermore, we evaluated if the cell death mechanism induced by our treatments was immunogenic cell death. To evaluate antitumor efficacy, we assessed tumor volume and production of tumor necrosis factor-α, nuclear factor κ B (both by ELISA), and nitric oxide levels (Nitrate/Nitrite colorimetric assay kit); for immunogenic cell death, we evaluated the release of danger-associated molecular patterns using immunohistochemistry and flow cytometry, as well as an in vivo challenge. Our results showed that the combination of colloidal silver and sodium dichloroacetate is more effective than each treatment alone and that the antitumor mechanism is not through immunogenic cell death. Furthermore, this study can broadly contribute to the development of dichloroacetate-loaded silver nanoparticles and to the design targeted pharmacological formulations to fight melanoma as well as other types of cancer.
ISSN:1687-4110
1687-4129
DOI:10.1155/2020/3741019