Determination of density gradients in isodensity equilibrium ultracentrifugation

Formulas are developed to convert conventional activity coefficients of a binary solution to a density scale of concentration. These enable precise calculation of the effect of activity coefficient variation with concentration on the density gradient at equilibrium in an ultracentrifuge. Results are...

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Bibliographic Details
Published inArchives of biochemistry and biophysics Vol. 87; no. 2; pp. 289 - 292
Main Author Trautman, Rodes
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.1960
Subjects
Chemistry Techniques, Analytical
Old Medline
Ultracentrifugation
Viruses - chemistry
CHEMISTRY, ANALYTICAL
VIRUSES/chemistry
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Summary:Formulas are developed to convert conventional activity coefficients of a binary solution to a density scale of concentration. These enable precise calculation of the effect of activity coefficient variation with concentration on the density gradient at equilibrium in an ultracentrifuge. Results are given for CsCl at 25 °C. One value is verified from the displacement of a density marker in simultaneous experiments at slightly different initial density.
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SourceType-Scholarly Journals-1
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ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(60)90174-0