Enantiomeric Resolution of Drugs and Metabolites in Polysaccharide- and Protein-Based Chiral Stationary Phases

Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under...

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Published inJournal of the Brazilian Chemical Society Vol. 13; no. 2; pp. 190 - 199
Main Authors Bonato, Pierina S., Bortocan, Renato, Gaitani, Cristiane M., Paias, Fernanda O., Iha, Maria H., Lima, Rodrigo P.
Format Journal Article
LanguageEnglish
Portuguese
Published Sociedade Brasileira de Química 2002
Subjects
CHEMISTRY, MULTIDISCIPLINARY
drugs
enantioselective analysis
metabolites
polysaccharide-based stationary phases
protein- based stationary phases
enantioselective analysis
polysaccharide-based stationary phases
drugs
metabolites
protein- based stationary phases
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Summary:Several chiral stationary phases based on polysaccharide derivatives and proteins were evaluated for the resolution of some chiral drugs and their metabolites. The polysaccharide-based stationary phases CHIRALCEL OD-H, CHIRALCEL OB-H, CHIRALCEL OJ, CHIRALPAK AD and CHIRALPAK AS were evaluated under normal phase conditions, using hexane:2-propanol or hexane:ethanol as mobile phase. Diethylamine and trifluoracetic acid were also added to improve peak shape. The CHIRALCEL OJ-R, CHIRALCEL OD-R and CHIRALCEL OD-H columns were evaluated under reversed-phase conditions, using acetonitrile:H2O or acetonitrile:NaClO4 solution. The protein- based stationary phases, CHIRAL AGP and ULTRON ES-OVM columns were used with mobile phases consisting of a buffer solution supplemented with an organic modifier. Among the polysaccharide-based stationary phases, CHIRALPAK AD provided better resolution for almost all drugs and metabolites studied. The ULTRON ES-OVM column was particularly suitable for the resolution of the four enantiomers of thioridazine-2-sulfoxide.
ISSN:0103-5053
1678-4790
DOI:10.1590/S0103-50532002000200010