Somatic antigens of pseudomonads: Structure of the O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247

• Published in: Carbohydrate research Vol. 306; no. 1; pp. 297 - 303
• Main Authors: Shashkov, Alexander S, Paramonov, Nikolay A, Veremeychenko, Stanislav P, Grosskurth, Horst, Zdorovenko, Galina M, Knirel, Yuriy A, Kochetkov, Nikolay K
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 1998; Elsevier
• Subjects: ( S)-3-Hydroxybutyrate; 2,4-Diamino-2,4,6-trideoxy- d-glucose; 2-Amino-2-deoxy- d-galacturonic acid; 3-Amino-3,6-dideoxy- d-glucose; Bacterial polysaccharide; Biochemistry & Molecular Biology; Carbohydrate Conformation; Carbohydrate Sequence; Chemistry; Chemistry, Applied; Chemistry, Organic; Gas Chromatography-Mass Spectrometry; Life Sciences & Biomedicine; Lipopolysaccharide; Magnetic Resonance Spectroscopy; Molecular Sequence Data; O Antigens - analysis; Oligosaccharides - chemistry; Physical Sciences; Pseudomonas fluorescens; Pseudomonas fluorescens - immunology; Science & Technology; 3-Amino-3,6-dideoxy- d-glucose; ( S)-3-Hydroxybutyrate; 2,4-Diamino-2,4,6-trideoxy- d-glucose; Lipopolysaccharide; 2-Amino-2-deoxy- d-galacturonic acid; Pseudomonas fluorescens; Bacterial polysaccharide; CHAIN; GLYCOSIDES; AERUGINOSA; (S)-3-hydroxybutyrate; bacterial polysaccharide; 3-amino-3,6-dideoxy-D-glucose; 2,4-diamino-2,4,6-trideoxy-D-glucose; lipopolysaccharide; 2-amino-2-deoxy-D-galacturonic acid; C-13 CHEMICAL-SHIFTS
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/S0008-6215(97)10048-9

The O-specific polysaccharide of Pseudomonas fluorescens biovar B, strain IMV 247, was studied by acid hydrolysis, GLC–MS and 1 H and 13 C NMR spectroscopy, including 1D and 2D NOE, 2D hybrid TOCSY and ROESY (TORO), and 2D H-detected heteronuclear multiple-bond correlation (HMBC) experiments. The polysaccharide was found to contain l-rhamnose, 3,6-dideoxy-3-[( S)-3-hydroxybutyramido]- d-glucose ( d-Qui3NHb), 2-acetamido-2,4,6-trideoxy-4-[( S)-3-hydroxybutyramido- d-glucose ( d-QuiNAc4NHb) and 2-acetamido-2-deoxy- d-galacturonic acid ( d-GalNAcA). Partial acid hydrolysis of the polysaccharide resulted in a non-reducing GalNAcA→QuiNAc4NHb disaccharide with the 3-hydroxybutyryl group glycosylated intramolecularly by the QuiN4N residue. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →4)- α- d-Gal pNAcA-(1→3)- α- d-Qui pNAc4NHb-(1→2)- β- d-Qui p3NHb-(1→2)- α- l-Rha p-(1→.