Binding of Tau Protein to the Ends of ex vivo Paired Helical Filaments

Human recombinant tau can bind to the end of isolated human paired helical filaments (PHF). The sequential binding of tau protein to PHF could result in an elongation of the previously polymerized PHF. However, we have observed that the elongation takes place in a different way on different types of...

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Bibliographic Details
Published inJournal of Alzheimer's disease Vol. 13; no. 2; pp. 177 - 185
Main Authors Santa-Maria, Ismael, Cuadros, Raquel, Moreno, Francisco J., Muñoz, Victor, Ávila, Jesús, Hernández, Félix
Format Journal Article
LanguageEnglish
Published London, England SAGE Publications 01.03.2008
Subjects
Alzheimer Disease - genetics
Alzheimer Disease - metabolism
Binding Sites
Carbocyanines
Fluorescent Antibody Technique
Fluorescent Dyes
Humans
Intermediate Filament Proteins - genetics
Microscopy, Electron
Microtubules - metabolism
Molecular Conformation
tau Proteins - genetics
tau Proteins - metabolism
Tubulin Modulators - metabolism
paired helical filaments
tau protein
Elongation
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Summary:Human recombinant tau can bind to the end of isolated human paired helical filaments (PHF). The sequential binding of tau protein to PHF could result in an elongation of the previously polymerized PHF. However, we have observed that the elongation takes place in a different way on different types of PHF. We have found that there are at least three populations of PHF. For one population, tau protein is able to bind to the ends of the filament and to elongate that filament. In the second PHF population, tau protein binds but does not elongates the filament. In the third, neither tau binding nor elongation was observed.
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ISSN:1387-2877
1875-8908
DOI:10.3233/JAD-2008-13208