One-step electrochemical approach of enzyme immobilization for bioelectrochemical applications
Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, and has led to important applications for biosensors, biofuel cells, and bioelectrocatalysis. Enzyme immobilization is the basis of enzymatic bioelectrochemistry, as immobilization itself determines...
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Published in | Synthetic metals Vol. 291; p. 117205 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier B.V
01.12.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Enzymatic bioelectrochemistry represents the marriage of electrochemistry and enzymatic biocatalysis, and has led to important applications for biosensors, biofuel cells, and bioelectrocatalysis. Enzyme immobilization is the basis of enzymatic bioelectrochemistry, as immobilization itself determines the enzyme/material interface and thus the electrochemical performance. Amongst the range of methods of enzyme immobilization, one-step electrochemical approaches feature rapid immobilization and good control over the processes, enabling partial or total use of the electrode surface. In this mini-review, we first briefly introduce the operating principles of bioelectrochemical applications based on enzyme modified electrodes. We then overview recent progress in utilizing conductive polymers, redox-active modified polymers, sol–gel silica and electrochemically assistant adsorption for enzyme immobilization via one-step electrochemical approaches. The use of conductive polymers for in situ enzyme immobilization is our major focus. Perspectives for future work are also described.
•One-step electrochemical approaches for enzyme immobilization are reviewed.•Emphasis is devoted to in situ enzyme immobilization with conductive polymers.•Perspectives are finally offered. |
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ISSN: | 0379-6779 1879-3290 |
DOI: | 10.1016/j.synthmet.2022.117205 |