Expression of a green tissue-specific 11 kDa proline-rich protein gene in bean in response to heavy metals

• Published in: Plant science (Limerick) Vol. 133; no. 1; pp. 47 - 56
• Main Authors: Yao Chai, Tuan, Didierjean, Luc, Burkard, Gérard, Genot, Geneviève
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 06.04.1998; Elsevier Science
• Subjects: abscisic acid; Agronomy. Soil science and plant productions; air temperature; amino acid sequences; Biological and medical sciences; complementary DNA; Drought; Economic plant physiology; Fundamental and applied biological sciences. Psychology; gene expression; Heat stress; heavy metals; leaves; mechanical damage; messenger RNA; Metabolism; Metabolism. Physicochemical requirements; Metal ions; molecular sequence data; molecular weight; Nitrogen metabolism and other ones (excepting carbon metabolism); nucleotide sequences; Nutrition. Photosynthesis. Respiration. Metabolism; open reading frames; Phaseolus vulgaris; Plant physiology and development; plant proteins; proline; protein synthesis; PRP; roots; salinity; stems; stress; ultraviolet radiation; Wounding; Wounding; Heat stress; Drought; PRP; Metal ions; Nucleotide sequence; Injury; Tissue specificity; Homology; Gene expression; Stress; Heavy metal; Regulation(control); Leguminosae; Complementary DNA; Gene; Phaseolus vulgaris; Dicotyledones; Thermal shock; Angiospermae; Isolation; Spermatophyta; Aminoacid sequence
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(98)00028-4

A cDNA clone encoding a proline-rich protein specifically expressed in green tissues of bean has been isolated. This clone contains an open reading frame of 288 nucleotides coding for a putative protein of 96 amino acids with an estimated molecular mass of 11 kDa. This protein, named PvPRP2, contains several specific amino acid motifs such as PPVYK and shows a high degree of sequence identity with the soybean SbPRP3. The PvPRP2 gene is constitutively expressed in stems and to a lesser extent in primary leaf tissue. In both tissues the cDNA probe hybridized to a 600-nucleotide transcript. In root-tissues, where no 600-nucleotide transcript was detectable, the probe hybridized to a transcript of about 900 bp which was not found in green tissues, thus suggesting that both roots and green tissues contain specific PRPs. In contrast to the green tissues genes, the expression of the root-gene is not altered by heavy metal stresses. While transcripts were at low levels in unstressed bean leaves, most of the abiotic stresses we have investigated (metals, wounding, drought, elevated temperature, UV, salt and ABA) triggered a strong response by increasing the expression of this gene.