Association of nuclear factor-kappa B1 (NF-kB1) Ins/Del gene polymorphism with Hepatitis C virus outcomes

• Published in: Journal of the Pakistan Medical Association Vol. 74; no. 10 (Supple-08); pp. S34 - S38
• Main Authors: Al-Salihy, Shaimaa Rahem, Al-Shawk, Refif Sabeeh, Al-Waysi, Safaa Abdul-Karim, Rasheed, Maarib Nazih
• Format: Journal Article
• Language: English
• Published: Pakistan Pakistan Medical Association 01.10.2024
• Subjects: Adult; Aged; Antiviral Agents - therapeutic use; Case-Control Studies; Female; Genetic Predisposition to Disease; Hepacivirus - genetics; Hepatitis C - genetics; Hepatitis C, Chronic - genetics; Humans; INDEL Mutation; Iraq; Male; Middle Aged; NF-kappa B p50 Subunit - genetics; Polymorphism, Genetic; Young Adult; Iraq; Antiviral, Gastroenterology, Hepaciviral, Thymine, Alleles, Homozygote, Hepatitis C, Chronic, Adenine, Guanine
• ISSN: 0030-9982; 0030-9982
• DOI: 10.47391/JPMA-BAGH-16-09

Objective: To explore the association of nuclear factor-kappa B1 polymorphism in the promotor area of the genewith hepatitis C virus infection outcomes.Method: The case-control study was conducted at the Hepatology and Gastroenterology Teaching Hospital,Baghdad, Iraq, from Dec 1, 2020, to Aug 30, 2021, and comprised individuals ages 20-68 years. Group 1 had patientswith persistent hepatitis C virus infection, group 2 had subjects with spontaneous hepatitis C virus clearance, group 3 had subjects treated with direct-acting antiviral drugs, and group 4 had healthy controls. Venous blood was collected for polymorphism genetic analysis of nuclear factor-kappa B1 insertion/deletion ATTG (Adenine-Thymine- Thymine-Guanine) at rs28362491 using a high-resolution melting technique. Data was analysed using SPSS 27.Results: Of the 88 subjects, there were 22(25%) in each of the 4 groups. Overall, there were 55(62.5%) females and33(37.5%) males, and 40(45.45%) were aged 20-39 years while 48(54.54%) were aged 40-68 years (p>0.05). The Insallele of rs28362491 was significantly more frequent in the patients than in controls (p=0.0053). The carriage of rs28362491 insertion/insertion and insertion/deletion genotypes, compared to wild-type homozygousdeletion/deletion, had a significantly higher risk of developing hepatitis C virus infection (p=0.0013). No association was found between rs28362491 and spontaneous hepatitis C virus clearance (p>0.05).Conclusion: The insertion allele of rs28362491 was found to be associated with increased susceptibility todeveloping hepatitis C virus infection.Key Words: Antiviral, Gastroenterology, Hepaciviral, Thymine, Alleles, Homozygote, Hepatitis C, Chronic, Adenine, Guanine.