Isolation, purification and characteristics of mononuclear phagocytes from human placentas

Methods for the isolation of mononuclear phagocytes from human placentas by digestion with collagenase or with trypsin are described. Mononuclear phagocytes were approximately 35% of the cells obtained. Preparations were enriched for mononuclear phagocytes by sequential density gradient centrifugati...

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Bibliographic Details
Published inJournal of immunological methods Vol. 56; no. 3; p. 305
Main Authors Wilson, C B, Haas, J E, Weaver, W M
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.1983
Subjects
Cell Count
Cell Separation - methods
Cells, Cultured
Culture Media
Female
Humans
Macrophages - enzymology
Macrophages - immunology
Macrophages - ultrastructure
Male
Microbial Collagenase - pharmacology
Monocytes - ultrastructure
Periodic Acid-Schiff Reaction
Placenta - cytology
Pregnancy
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Summary:Methods for the isolation of mononuclear phagocytes from human placentas by digestion with collagenase or with trypsin are described. Mononuclear phagocytes were approximately 35% of the cells obtained. Preparations were enriched for mononuclear phagocytes by sequential density gradient centrifugation over Ficoll-Hypaque and Percoll, adherence to plastic and removal of contaminating trophoblastic cells with brief trypsin exposure. Monolayers obtained by these methods were greater than 85% mononuclear phagocytes. These cells were predominantly of fetal origin; most were mature macrophages but up to 20% appeared to be recently derived from blood monocytes as determined by ultrastructural peroxidase cytochemistry.
ISSN:0022-1759
DOI:10.1016/S0022-1759(83)80020-9