Lactobacillus plantarum NK3 and Bifidobacterium longum NK49 Alleviate Bacterial Vaginosis and Osteoporosis in Mice by Suppressing NF- κ B-Linked TNF- α Expression

• Published in: Journal of medicinal food Vol. 22; no. 10; p. 1022
• Main Authors: Kim, Da-Eun, Kim, Jeon-Kyung, Han, Sang-Kap, Jang, Se-Eun, Han, Myung Joo, Kim, Dong-Hyun
• Format: Journal Article
• Language: English
• Published: United States 01.10.2019
• Subjects: Animals; Bifidobacterium longum - physiology; Cells, Cultured; Feces - microbiology; Female; Fermented Foods and Beverages - microbiology; Gardnerella vaginalis - pathogenicity; Humans; Lactobacillus plantarum - physiology; Macrophages - metabolism; Mice; Mice, Inbred C57BL; NF-kappa B - metabolism; Osteoporosis - therapy; Ovariectomy; Probiotics - therapeutic use; Tumor Necrosis Factor-alpha - metabolism; Vaginosis, Bacterial - therapy; Bifidobacterium longum; Lactobacillus plantarum; osteoporosis; bacterial vaginosis; obesity
• ISSN: 1557-7600
• DOI: 10.1089/jmf.2019.4419

Excessive expression of TNF- worsens bacterial vaginosis (BV) and osteoporosis. Therefore, to understand whether probiotics could alleviate vaginosis and osteoporosis, we isolated anti-inflammatory NK3 and NK49 from kimchi and human fecal lactic acid bacteria collection and examined their effects on (GV)-induced vaginosis and ovariectomy-induced osteoporosis in female mice. Oral gavage of NK3 and/or NK49 significantly alleviated GV-induced vaginosis; these inhibited NF- B activation and TNF- expression in the vagina and uterus, and decreased the GV population in the vagina. Furthermore, treatment with NK3 and/or NK49 alleviated ovariectomy-induced osteoporosis and obesity; these increased blood calcium, phosphorus, and osteocalcin levels and suppressed body weight. GV-induced vaginosis and ovariectomy increased colonic myeloperoxidase activity, TNF- expression, and fecal Proteobacteria population. NK3 and/or NK49 treatments reduced TNF- expression and NF- B activation in the colon. NK3 and NK49 treatment also restored GV- or ovariectomy-disrupted gut microbiota composition. In conclusion, NK3 and NK49 may simultaneously alleviate BV and osteoporosis by suppressing NF- B-linked TNF- expression through the regulation of gut microbiota population.