Dietary Selenium Deficiency or Excess Reduces Sperm Quality and Testicular mRNA Abundance of Nuclear Glutathione Peroxidase 4 in Rats

• Published in: The Journal of nutrition Vol. 147; no. 10; pp. 1947 - 1953
• Main Authors: Zhou, Ji-Chang, Zheng, Shijie, Mo, Junluan, Liang, Xiongshun, Xu, Yuanfei, Zhang, Huimin, Gong, Chunmei, Liu, Xiao-Li, Lei, Xin Gen
• Format: Journal Article
• Language: English
• Published: United States American Institute of Nutrition 01.10.2017
• Subjects: 8-Hydroxy-2'-Deoxyguanosine; Abundance; Animals; Body weight; Deficiency Diseases - blood; Deficiency Diseases - complications; Deficiency Diseases - metabolism; Deformation; Deoxyguanosine; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - blood; Diet; Glutathione; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Liver; Liver - metabolism; Male; mRNA; Nutrition Disorders - blood; Nutrition Disorders - complications; Nutrition Disorders - metabolism; Parameter sensitivity; Peroxidase; Phospholipid Hydroperoxide Glutathione Peroxidase; Quality; Rats; Rats, Sprague-Dawley; Ribonucleic acid; RNA; RNA, Messenger - metabolism; Rodents; Selenium; Selenium - blood; Selenium - deficiency; Selenium - metabolism; Selenoprotein P - metabolism; Semen; Sperm; Spermatozoa; Testes; Testis - metabolism; Vitamin deficiency; Weaning; glutathione peroxidase 4; transcript variant; dietary selenium; sperm quality; rodent; selenoprotein P
• ISSN: 0022-3166; 1541-6100
• DOI: 10.3945/jn.117.252544

Glutathione peroxidase (GPX) 4 and selenoprotein P (SELENOP) are abundant, and several variants are expressed in the testis. We determined the effects of dietary selenium deficiency or excess on sperm quality and expressions of GPX4 and SELENOP variants in rat testis and liver. After weaning, male Sprague-Dawley rats were fed a Se-deficient basal diet (BD) for 5 wk until they were 9 wk old [mean ± SEM body weight (BW) = 256 ± 5 g]. They were then fed the BD diet alone (deficient) or with 0.25 (adequate), 3 (excess), or 5 (excess) mg Se/kg for 4 wk. Testis, liver, blood, and semen were collected to assay for selenoprotein mRNA and protein abundances, selenium concentration, GPX activity, 8-hydroxy-deoxyguanosine concentration, and sperm quality. Dietary selenium supplementations elevated ( < 0.05) tissue selenium concentrations and GPX activities. Compared with those fed BD + 0.25 mg Se/kg, rats fed BD showed lower ( < 0.05) BW gain (86%) and sperm density (57%) but higher ( < 0.05) plasma 8-hydroxy-deoxyguanosine concentrations (189%), and nonprogressive sperm motility (4.4-fold). Likewise, rats fed BD + 5 mg Se/kg had ( = 0.06) lower BW gain and higher (1.9-fold) sperm deformity rates than those in the selenium-adequate group. Compared with the selenium-adequate group, dietary selenium deficiency (BD) or excess (BD + 3 or 5 mg Se/kg) resulted in 45-77% lower ( < 0.05) nuclear ( ) mRNA abundance in the testis. Rats fed BD had lower ( < 0.05) mRNA levels of 2 variants in both testis and liver than those in the other groups. Testicular SELENOP was 155-170% higher ( < 0.05) in rats fed BD + 5 mg Se/kg and hepatic c/mGPX4 was 13-15% lower ( < 0.05) in rats fed BD than in the other groups. The mRNA abundance of rat testicular nGPX4 responded to dietary selenium concentrations in similar ways to sperm parameters and may be used as a sensitive marker to assess appropriate Se status for male function.