Establishment and characterization of a cell line, MT-213-VGH, isolated from a mixed müllerian tumor of the uterus

• Published in: Acta cytologica Vol. 45; no. 5; p. 683
• Main Authors: Chao, K C, Wang, P H, Chang, C C, Lai, C R, Ng, H T
• Format: Journal Article
• Language: English
• Published: Switzerland 01.09.2001
• Subjects: Antineoplastic Agents - pharmacology; Cell Culture Techniques - methods; Female; Humans; Middle Aged; Mixed Tumor, Mullerian - drug therapy; Mixed Tumor, Mullerian - metabolism; Mixed Tumor, Mullerian - pathology; Staining and Labeling - methods; Tumor Cells, Cultured; Uterine Neoplasms - drug therapy; Uterine Neoplasms - metabolism; Uterine Neoplasms - pathology
• ISSN: 0001-5547; 1938-2650
• DOI: 10.1159/000328288

To establish a cell line from a woman with malignant mixed müllerian tumor of the uterus and to examine the biologic properties of this cell line (MT-213-VGH). Cells were cloned by the limiting dilution method. Histologic staining of mixed müllerian (mesodermal) tumor (MMMT) cells was performed with May-Grünwald-Giemsa and hematoxylin and eosin stain. After more than 20 passages, cells were used to estimate the population-doubling time and colony-forming efficiency of MMMT cells. The cell line exhibited considerable variation in the degree of sensitivity to diverse chemotherapy drugs in vitro. MMMT cells containing antigens for vimentin and myoglobin were detected, but those for CA-125, carcinoembryonic antigen, cytoskeleton, desmin, epithelial membrane antigen and fibronectin were not found. In addition, MT-213-VGH cells contained a mucinous substance; its chromosome model number is 45. This cell line showed differential sensitivities to chemotherapeutic agents, such as bleomycin, cisplatin, 5-fluorouracil and vinblastine. The establishment and availability of the number cell line MT-213-VGH for a malignant mixed müllerian tumor of the uterus should assist in research on new methods of managing this type of gynecologic cancer.