Structure and expression of the zebrafish mest gene, an ortholog of mammalian imprinted gene PEG1/ MEST
PEG1/MEST is a paternally expressed gene in placental mammals. Here, we report identification of zebrafish ( Danio rerio) gene mest, an ortholog of mammalian PEG1/MEST. Zebrafish mest encodes a polypeptide of 344 amino acids and shows a significant similarity to mammalian orthologs. Zebrafish mest i...
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Published in | Biochimica et biophysica acta Vol. 1731; no. 2; pp. 125 - 132 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
10.11.2005
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Subjects | |
Online Access | Get full text |
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Summary: | PEG1/MEST is a paternally expressed gene in placental mammals. Here, we report identification of zebrafish (
Danio rerio) gene
mest, an ortholog of mammalian
PEG1/MEST. Zebrafish
mest encodes a polypeptide of 344 amino acids and shows a significant similarity to mammalian orthologs. Zebrafish
mest is present as a single copy in the zebrafish genome and is closely linked to
copg2 as in mammals. It is notable that 10 of 11 intron positions in
mest are conserved among mammalian
PEG1/MEST genes, indicating that the genomic organization and linkage between
mest and
copg2 loci was established in ancient vertebrates. Zebrafish
mest is expressed in blastula, segmentation, and larval stages, exhibiting gradually increased expression as the development proceeds. Allelic expression analysis in hybrid larvae shows that both parental alleles are transcribed. We also observed one-codon alternative splicing involving an alternative usage of the two consecutive splice acceptors of intron 1, generating two protein isoforms with different lengths of a single amino acid. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0167-4781 0006-3002 1879-2634 |
DOI: | 10.1016/j.bbaexp.2005.09.004 |