Cell cycle-dependent expression of Ki-67 antigen in human melanoma cells subjected to irradiation and/or hyperthermia

The proliferation of human melanoma cells in vitro during the first 3 days after irradiation and/or hyperthermia was followed by two-parameter flow cytometry combining cell cycle analysis on the basis of DNA content with Ki-67 antibody labeling. It was found that cells arrested or delayed in the S a...

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Bibliographic Details
Published inRadiation research Vol. 143; no. 1; p. 98
Main Authors Zölzer, F, Streffer, C
Format Journal Article
LanguageEnglish
Published United States 01.07.1995
Subjects
Cell Cycle - radiation effects
Flow Cytometry
Hot Temperature
Humans
Ki-67 Antigen
Melanoma - immunology
Melanoma - pathology
Neoplasm Proteins - biosynthesis
Nuclear Proteins - biosynthesis
Tumor Cells, Cultured
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Summary:The proliferation of human melanoma cells in vitro during the first 3 days after irradiation and/or hyperthermia was followed by two-parameter flow cytometry combining cell cycle analysis on the basis of DNA content with Ki-67 antibody labeling. It was found that cells arrested or delayed in the S and G2 phases of the cell cycle were Ki-67-positive in spite of the antigen's very short half-life. Thus Ki-67 staining failed to reflect those changes in cell proliferation which typically occur in the course of a fractionated radiotherapy as well as those expected in the case of hyperthermia or a combined treatment.
ISSN:0033-7587
DOI:10.2307/3578931