Comparative identification of Candida species isolated from animals using phenotypic and PCR-RFLP methods

The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. k...

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Bibliographic Details
Published inBulletin of the Veterinary Institute in Puławy Vol. 58; no. 2; pp. 219 - 222
Main Authors Nadăş, George Cosmin, Kalmár, Zsuzsa, Taulescu, Marian Aurel, Chirilă, Flore, Bouari, Cosmina Maria, Răpuntean, Sorin, Bolfa, Pompei, Fiţ, Nicodim Iosif
Format Journal Article
LanguageEnglish
Published Pulawy Sciendo 01.01.2014
De Gruyter Poland
Subjects
Candida
Candida krusei
Chromatic Candida
Genotyping
identification
PCR-RFLP
Polymerase chain reaction
Restriction fragment length polymorphism
Romania
Strains (organisms)
test API 20 C AUX system
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Summary:The aim of this study was to identify 58 Candida sp. strains isolated from animals using the Chromatic Candida test, the API 20 C AUX system, and polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP). The Chromatic Candida test was able to identify only C. albicans and C. krusei. The API 20 C AUX system and PCR-RFLP had similar specificity for the identification of Candida strains. In case of both methods, Candida albicans was the most frequently isolated species - 22 (37.93%) strains, followed by Candida krusei - 17 (29.31%) strains, Candida famata - 10 (17.24%) strains, Candida parapsilosis - five (8.62%) strains, and Candida kefyr - four (6.89%) strains. PCR-RFLP represents a reliable, quick and relatively inexpensive genotyping method, recommended for rapid identification of Candida spp.
ISSN:2300-3235
2450-7393
2300-3235
2450-8608
DOI:10.2478/bvip-2014-0032