On the nucleic acids of green and colorless Euglena gracilis: Isolation and composition of deoxyribonucleic acid and of transfer ribonucleic acid

The isolation and composition of two species of nucleic acid, DNA and transfer RNA, from Euglena gracilis are described. Preparations from green and colorless cells of this organism did not differ in nucleotide composition. Several minor componentswere identified in hydrolysates of the transfer RNA....

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Published inBiochimica et biophysica acta Vol. 61; no. 3; pp. 340 - 345
Main Authors Brawerman, George, Hufnagel, Dorothy Anne, Chargaff, Erwin
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 17.09.1962
Subjects
DNA
Euglena
Euglena gracilis
Nucleic Acids
Nucleotides
Old Medline
RNA
RNA, Transfer
NUCLEOTIDES
RNA
EUGLENA
DNA
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Summary:The isolation and composition of two species of nucleic acid, DNA and transfer RNA, from Euglena gracilis are described. Preparations from green and colorless cells of this organism did not differ in nucleotide composition. Several minor componentswere identified in hydrolysates of the transfer RNA. The DNA was found to contain more than 2 mole per cent of 5-methylcytosine and nearly equimolar amounts of the 4 major nitrogenous constituents. The sugar component of the DNA appears to be 2-deoxyribose.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0926-6550
0006-3002
1878-2256
DOI:10.1016/0926-6550(62)90134-2