CircRNA hsa_ circ_0009022 inhibits colorectal cancer progression by sponging miR-576–5p and complexing with FMRP

• Published in: Tissue & cell Vol. 95; p. 102924
• Main Authors: Guo, Jingyu, Wang, Yongxia, Yang, Yang, Feng, Shuang, Cai, Jinlong, Fan, Shangqian, Wang, Jingnan, Li, Na, Qian, Xinlai, Zhu, Huifang
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.08.2025
• Subjects: Animals; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Colorectal cancer; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Disease Progression; EMT; Epithelial-Mesenchymal Transition - genetics; Female; FMRP; Fragile X Mental Retardation Protein - genetics; Fragile X Mental Retardation Protein - metabolism; Gene Expression Regulation, Neoplastic; Hsa_ circ_0009022; Humans; Male; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; MiR-576–5p; Neoplasm Invasiveness; RNA, Circular - genetics; RNA, Circular - metabolism; Hsa_ circ_0009022; MiR-576–5p; FMRP; EMT; Colorectal cancer
• ISSN: 0040-8166; 1532-3072; 1532-3072
• DOI: 10.1016/j.tice.2025.102924

Colorectal cancer (CRC) is a type of malignant tumor that poses a serious risk to people's health.According to the current study, abnormal expression of circular RNA (circ RNA) is closely related to the occurrence and development of CRC. However, its specific molecular regulation mechanism remains unclear. Our research found that the expression of hsa_ circ_0009022 (circ_009022) is negatively correlated with the invasion and metastasis of CRC. Functionally, interference with circ_0009022 can promote the proliferation and migration of CRC cells, while overexpression of circ_0009022 inhibits the proliferation and migration of CRC cells. Mechanically, circ_0009022 can up-regulate the transcription factor TFAP2A and down-regulate the expression of Vimentin by binding with miR-576–5p and FMRP respectively, thereby inhibiting the EMT process and inhibiting the migration and metastasis of CRC. This subject intends to further explore specific mechanism of circ_0009022 on inhibition of CRC through a variety of experiments in vitro and in vivo. This study is expected to provide a new therapeutic target for CRC on clinical translational medicine. •Hsa_ circ_0009022 is negatively correlated with the invasion and metastasis of colorectal cancer.•Hsa_ circ_0009022 exerts a tumor-suppressive function of colorectal cancer in vitro and in vivo.•Hsa_ circ_0009022 serves as a ceRNA for miR-576–5p to inhibit the EMT of colorectal cancer.•Hsa_ circ_0009022 binds FMRP protein to inhibit the EMT phenotype of colorectal cancer cells.