Prostate specific antigen reverse transcriptase-polymerase chain reaction assay in preoperative staging of prostate cancer

• Published in: The Journal of urology Vol. 158; no. 5; p. 1870
• Main Authors: Ignatoff, J M, Oefelein, M G, Watkin, W, Chmiel, J S, Kaul, K L
• Format: Journal Article
• Language: English
• Published: United States 01.11.1997
• Subjects: Aged; Humans; Logistic Models; Male; Middle Aged; Neoplasm Staging; Polymerase Chain Reaction; Predictive Value of Tests; Preoperative Care; Prospective Studies; Prostate-Specific Antigen - analysis; Prostate-Specific Antigen - genetics; Prostatic Neoplasms - blood; Prostatic Neoplasms - pathology; RNA - analysis; Sensitivity and Specificity
• ISSN: 0022-5347
• DOI: 10.1016/S0022-5347(01)64150-8

Extracapsular extension of prostate cancer occurs in a significant number of men believed to have clinically localized disease. We report the ability of the reverse transcriptase-polymerase chain reaction (RT-PCR) assay to predict preoperatively the pathological stage of cases of clinically localized prostate cancer. Since October 1994, 82 consecutive men with clinically localized prostate cancer had a venous blood RT-PCR assessment before radical retropubic prostatectomy. The extracted ribonucleic acid was reverse transcribed, amplified and the amplicon identity confirmed by prostate specific antigen (PSA) directed probe hybridization. An additional 31 patients were enrolled to provide appropriate positive (T + Nx/1M2) and negative (human female and benign prostatic hyperplasia) controls. Histological examination of the entire prostatectomy specimen was performed. Positive RT-PCR assay results correlated significantly with skeletal metastases and elevated levels of serum PSA but they did not significantly improve our ability to identify prospectively patients with extracapsular extension over traditional predictors (serum PSA, Gleason score). The role of molecular techniques in prostate cancer evaluation and prognosis continues to emerge. However, in our study we demonstrate no significant advantage in preoperative staging of prostate cancer using RT-PCR assay with PSA primers.