Ultrasound-assisted extraction of Ca, K and Mg from in vitro citrus culture

An ultrasound extraction procedure for Ca, K and Mg from in vitro plant cultures is proposed, comparing cultures of different embryogenic levels of Citrus sinensis and Citrus limonia, employing ultrasound energy. Parameters related to metals extraction, such as plant material sampling, acid concentr...

Full description

Saved in:
Bibliographic Details
Published inJournal of the Brazilian Chemical Society Vol. 14; no. 3; pp. 470 - 474
Main Authors Arruda, Sandra C. C., Rodriguez, Adriana P. M., Arruda, Marco A. Z.
Format Journal Article
LanguageEnglish
Published Sociedade Brasileira de Química 01.05.2003
Subjects
Ca, K and Mg determination
CHEMISTRY, MULTIDISCIPLINARY
FAAS
FAES
ultrasound extraction
Ca, K and Mg determination
FAES
ultrasound extraction
FAAS
Online AccessGet full text

Cover

More Information
Summary:An ultrasound extraction procedure for Ca, K and Mg from in vitro plant cultures is proposed, comparing cultures of different embryogenic levels of Citrus sinensis and Citrus limonia, employing ultrasound energy. Parameters related to metals extraction, such as plant material sampling, acid concentration and sonication time were investigated. For accuracy check, the proposed ultrasound extraction procedure was compared with a microwave-assisted digestion procedure and no differences in the results were verified at 95% of the confidence level. With this simple and accurate extraction procedure, it was possible to determine differences in Ca, K and Mg concentrations during Citrus embryo formation/development and between cultures (embryogenic and non-embryogenic). Finally, the ultrasound extraction method demonstrated to be an excellent alternative for handless sampling and operational costs.
ISSN:0103-5053
1678-4790
DOI:10.1590/S0103-50532003000300023