Purification and characterization of two distinct carboxymethylcellulases of Paecilomyces sp

• Published in: Bioresource technology Vol. 66; no. 3; pp. 231 - 234
• Main Authors: Okolo, J.C., Obi, S.K.C., Odibo, F.J.C.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.1998; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Carboxymethylcellulase; Enzyme engineering; Fermentation; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; lignocellulosic wastes; Methods. Procedures. Technologies; Production of selected enzymes; purification; rice hull; rice hulls; Use of agricultural and forest wastes. Biomass use, bioconversion; Carboxymethylcellulase; purification; rice hull; Culture medium; Purification; Enzyme; Lignocellulosics; Rice by product; Molecular weight property relation; Vegetal waste; Fungi; Paecilomyces; Enzymatic activity; Cellulase; Glycosidases; Biotransformation; Microorganism culture; Hydrolases; Fungi Imperfecti; O-Glycosidases; Physicochemical properties; Thallophyta
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/S0960-8524(98)00053-4

Two distinct forms of carboxymethylcellulase (CMCases) were produced by Paecilomyces sp. in a medium containing 5% (w/v) rice hull as the carbon source. CMCases I and II were purified 13·2- and 127·3-fold, respectively, by acetone precipitation, ion-exchange chromatography on DEAE-Sephadex A-50 and gel filtration on Sephadex G-200. Both enzymes were optimally active at 60°C and retained 100% of their original activity at 50°C for 2 h. They were also maximally active at pH 4·0 and stable between pH 4 and 5. CMCase I was activated by Ca 2+, while CMCase II was activated by Ba 2+. Both enzymes were, however, totally inhibited by Zn 2+, Hg 2+, glycine and EDTA. CMCase I and II had K m values of 1·42 and 0·91%, respectively, for carboxy-methylcellulose. The relative molecular weights were 55 and 42 KDa for CMCases I and II, respectively.