Production of amino acids by Azotobacter vinelandii and Azotobacter chroococcum with phenolic compounds as sole carbon source under diazotrophic and adiazotrophic conditions

• Published in: Amino acids Vol. 28; no. 4; pp. 421 - 425
• Main Authors: Revillas, J J, Rodelas, B, Pozo, C, Martínez-Toledo, M V, López, J González
• Format: Journal Article
• Language: English
• Published: Austria Springer Nature B.V 01.06.2005
• Subjects: Amino acids; Amino Acids - biosynthesis; Azotobacter; Azotobacter vinelandii - growth & development; Bacteria; Carbon; Hydroxybenzoates - pharmacology; Media; Microbiology; Sodium; Soil Microbiology; Sole; Species Specificity; Strain; Succinic Acid - pharmacology
• ISSN: 0939-4451; 1438-2199
• DOI: 10.1007/s00726-004-0153-x

Azotobacter vinelandii strain ATCC 12837 and Azotobacter chroococcum strain H23 (CECT4435) were tested to grow in N-free or NH(4)Cl amended chemically defined media, with protocatechuic acid or sodium p-hydroxybenzoate as sole carbon (C) sources at a concentration of 2 mmol/L. Both substrates supported grow at similar rates than bacteria grown in control media amended with 2 mmol/L sodium succinate as C source. The two strains produced aspartic acid, serine, glutamic acid, glycine, hystidine, threonine, arginine, alanine, proline, cysteine, tyrosine, valine, methionine, lysine, isoleucine, leucine and phenylalanine after 72 h of growth in chemically defined media with 2 mmol/L of phenolic compounds or sodium succinate as sole C source amended or unamended with 0.1% (w/v) NH(4)Cl. Qualitative and quantitative production of all amino acids was not affected by the use of different C and N substrates.