Influence of iron on oxidation of reduced nicotinamide- adenine-dinucleotide phosphate in rat-liver microsomes

1. 1. When Fe 2+ was added to a microsome suspension prepared from rat liver, there was an uptake of O 2 and a concomitant lipid peroxidation. These reactions were uninfluenced by the addition of ADP or NADPH 2. 2. 2. The addition of Fe 3+ to a liver-microsomal suspension increased O 2 uptake and li...

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Published inBiochimica et biophysica acta Vol. 97; no. 3; pp. 416 - 421
Main Authors Beloff-Chain, Anne, Serlupi-Crescenzi, G., Catanzaro, Raffaella, Venettacci, Domenica, Balliano, Marta
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 08.03.1965
Subjects
Adenine
Adenine Nucleotides
Antimycin A
Cytochromes
Edetic Acid
Iron
Lipid Metabolism
Liver - cytology
Metabolism
Microsomes
Microsomes, Liver
NADP
Niacinamide
OldMedline
Peroxides
Pharmacology
Phosphates
Polarography
Rats
Spectrophotometry
EXPERIMENTAL LAB STUDY
LIVER CYTOLOGY
POLAROGRAPHY
RATS
IRON
EDTA
ADENINE NUCLEOTIDES
SPECTROPHOTOMETRY
CYTOCHROMES
METABOLISM
PEROXIDES
LIPID METABOLISM
ANTIMYCIN A
MICROSOMES
PHARMACOLOGY
NADP
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Summary:1. 1. When Fe 2+ was added to a microsome suspension prepared from rat liver, there was an uptake of O 2 and a concomitant lipid peroxidation. These reactions were uninfluenced by the addition of ADP or NADPH 2. 2. 2. The addition of Fe 3+ to a liver-microsomal suspension increased O 2 uptake and lipid peroxidation in the presence of ADP and NADPH 2. 3. 3. Ethylenediaminetetraacetate and antimycin A inhibited O 2 uptake by rat- liver microsomes in the presence of NADPH 2 and ADP, and this inhibition was reversed by the addition of Fe 3+. 4. 4. The supernatant obtained after separating the microsomes by centrifugation at 105000 × g, inhibited O 2 uptake by the microsomes in the presence of NADPH 2 and low concentrations of ADP (2 μmoles/ml), and this inhibition was reversed by the addition of ADP (a further 2 μmoles/ml). 5. 5. Cytochrome b 5 was reduced by addition of NADPH 2 in the presence of antimycin A which blocked O 2 uptake. The cytochrome was re-oxidized on addition of Fe 3+ and ADP together. 6. 6. A tentative scheme for the participation of iron in the oxidation of NADPH 2 in rat-liver microsomes is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(65)90152-2