Determination of tilmicosin residues in cow and sheep milk by liquid chromatography
This method was developed and validated to detect and quantitate tilmicosin residues in cow milk over a range of 0.010-10 microg/mL, and in sheep milk over a range of 0.025-0.5 microg/mL. The procedure involves extracting the milk sample with acetonitrile and using a C18 cartridge to perform a solid...
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Published in | Journal of AOAC International Vol. 83; no. 3; p. 555 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
01.05.2000
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Subjects | |
Online Access | Get more information |
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Summary: | This method was developed and validated to detect and quantitate tilmicosin residues in cow milk over a range of 0.010-10 microg/mL, and in sheep milk over a range of 0.025-0.5 microg/mL. The procedure involves extracting the milk sample with acetonitrile and using a C18 cartridge to perform a solid-phase extraction cleanup of the extract. A reversed-phase gradient liquid chromatography method with detection at 280 nm is used to separate the tilmicosin from matrix components in a 30 min run time. The limit of quantitation of the method is 0.010 microg/mL for cow milk, and 0.025 microg/mL for sheep milk. Average percentage recoveries for milk samples ranged from 82 to 94%. Percentage relative standard deviation values ranged from 3.1 to 17.2%. |
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ISSN: | 1060-3271 |
DOI: | 10.1093/jaoac/83.3.555 |