Expression analysis of miR-221-3p and its target genes in horses

• Published in: Genes & genomics Vol. 41; no. 4; pp. 459 - 465
• Main Authors: Kim, So-Won, Jo, Ara, Im, Jennifer, Lee, Hee-Eun, Kim, Heui-Soo
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.04.2019; Springer Nature B.V; 한국유전학회
• Subjects: Animal Genetics and Genomics; Animals; Biomedical and Life Sciences; CDKN1C protein; Cerebellum; Cerebellum - metabolism; Chromosome 3; Conserved Sequence; Cyclin-Dependent Kinase Inhibitor p57 - genetics; Cyclin-Dependent Kinase Inhibitor p57 - metabolism; Evolution, Molecular; Evolutionary conservation; Gene expression; Genetic diversity; Horses - genetics; Human Genetics; Life Sciences; Medulla oblongata; Medulla Oblongata - metabolism; Microbial Genetics and Genomics; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Non-coding RNA; Nucleotides; Plant Genetics and Genomics; Polymerase chain reaction; Research Article; Spleen; Spleen - metabolism; 생물학; Quantitative polymerase chain reaction (qPCR); Expression analysis; Micro RNA 221-3p; CDKN1C; Equus caballus
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-018-00778-3

Background A microRNA (miRNA) is a small non-coding RNA (ncRNA) approximately 20 nucleotides long and it affects gene expression through mRNA cleavage or translational repression. Horses ( Equus caballus ) have been domesticated and bred to enhance their speed for racing. It has been studied extensively with genetic diversity, origins and evolution. Objectives We examined expression patterns of miR-221-3p and its target gene CDKN1C in various horse tissues. Methods We used bioinformatic tools to examine target gene, seed region and evolutionary conservation of miR-221-3p. The expression patterns of miR-221-3p and its target gene CDKN1C were analyzed by quantitative polymerase chain reaction (qPCR). Results Among eight tissues of horse, miR-221-3p was highly expressed in cerebellum and spleen. On the other hand, only medulla was highly expressed in CDKN1C gene. Conclusion Our study provides expression data of miR-221-3p and CDKN1C gene in horse and suggests the fundamental information for future studies in relation to functional importance.