Photoirradiated caffeic acid as an antimicrobial treatment for fresh produce

The antimicrobial efficacy of 400 nm photoirradiated caffeic acid (CA, 5 mM) was evaluated against Escherichia coli O157:H7 and Listeria innocua. A stronger antimicrobial effect was observed on E. coli than on L. innocua where the combined treatment resulted in 4 and 1 log(CFU/mL) reductions, respec...

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Bibliographic Details
Published inFEMS microbiology letters Vol. 365; no. 13
Main Authors Gilbert, Andrea R, Alborzi, Solmaz, Bastarrachea, Luis J, Tikekar, Rohan V
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.07.2018
Subjects
Antiinfectives and antibacterials
Antimicrobial agents
Caffeic acid
Combined treatment
Damage
E coli
Fluorescence
Food safety
Iodides
Listeria
Listeria innocua
Membranes
Microbiology
Microorganisms
Propidium iodide
Species
Spinach
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Summary:The antimicrobial efficacy of 400 nm photoirradiated caffeic acid (CA, 5 mM) was evaluated against Escherichia coli O157:H7 and Listeria innocua. A stronger antimicrobial effect was observed on E. coli than on L. innocua where the combined treatment resulted in 4 and 1 log(CFU/mL) reductions, respectively. The treatment's effects on cellular metabolism (resazurin assay), uptake of CA (fluorescence technique) and membrane damage (propidium iodide assay) were studied in both species. CA uptake increased in both species, but membrane damage was only observed in E. coli O157:H7. The treatment had minimal impact on metabolic activity in both species. The treatment applied to the surface of spinach leaves was found to be effective against E. coli O157:H7. The novel treatment proposed in this study has the potential to improve the microbial food safety of fresh produce.
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ISSN:1574-6968
0378-1097
1574-6968
DOI:10.1093/femsle/fny132