Novel intracellular antibiotic delivery system against Staphylococcus aureus : cloxacillin-loaded poly(d,l-lactide-co-glycolide) acid nanoparticles

First, to compare minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin susceptible (MSSA) and resistant (MRSA) and second, to assess NP antimicrobial activity against intracellular . Poly(d,l-lactide-co-glycolide) acid (PLGA)-N...

Full description

Saved in:
Bibliographic Details
Published inNanomedicine (London, England) Vol. 15; no. 12; pp. 1189 - 1203
Main Authors Lacoma, Alicia, Usón, Laura, Mendoza, Gracia, Sebastián, Victor, Garcia-Garcia, Esther, Muriel-Moreno, Beatriz, Domínguez, Jose, Arruebo, Manuel, Prat, Cristina
Format Journal Article
LanguageEnglish
Published England Future Medicine Ltd 01.05.2020
Subjects
Antibiotics
Antimicrobial agents
Bacteria
Fourier transforms
Microscopy
Nanoparticles
Pathogens
Staphylococcus infections
Tuberculosis
Vaccines
United States > US
antimicrobial
nanoparticle
intracellular infection
drug delivery
Staphylococcus aureus
Online AccessGet full text

Cover

More Information
Summary:First, to compare minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin susceptible (MSSA) and resistant (MRSA) and second, to assess NP antimicrobial activity against intracellular . Poly(d,l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP. For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2019-0371