Characterization by polyacrylamide gel electrophoresis of whole-cell proteins of some Bacillus thuringiensis subsp. israelensis strains isolated in Brazil

Of 14 strains of Bacillus thuringiensis selected from 956 isolates from soil samples from Brazil, 12 were toxic to larvae of Aedes fluviatilis and two were nontoxic. Nine of the 14 strains were serotyped as subspecies israelensis (serotype 14), one as subspecies kurstaki (serotype 3a 3b) one as subs...

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Bibliographic Details
Published inSystematic and applied microbiology Vol. 17; no. 1; pp. 104 - 107
Main Authors Kaji, D.A, Rosato, Y.B, Canhos, V.P, Priest, F.G
Format Journal Article
LanguageEnglish
Published 1994
Subjects
aedes fluviatilis
Bacillus thuringiensis
Bacillus thuringiensis subsp. israelensis
Bacillus thuringiensis subsp. kurstaki
bacillus thuringiensis subsp. morissoni
bacterial proteins
chemotaxonomy
larvae
soil bacteria
strains
toxicity
Brazil
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Summary:Of 14 strains of Bacillus thuringiensis selected from 956 isolates from soil samples from Brazil, 12 were toxic to larvae of Aedes fluviatilis and two were nontoxic. Nine of the 14 strains were serotyped as subspecies israelensis (serotype 14), one as subspecies kurstaki (serotype 3a 3b) one as subspecies morrisoni (serotype 8a 8b) and three did not agglutinate any antisera. Electrophoresis of whole cell proteins showed that all subsp. israelensis strains formed a homogeneous group which included two non-typable toxic strains, and could be readily distinguished from reference strains toxic for lepidoptera or coleoptera.
ISSN:0723-2020
1618-0984
DOI:10.1016/S0723-2020(11)80037-8