Stability of domoic acid in saline dosing solutions

Studies designed to assess the effects of repeated low doses of domoic acid require an assessment of its stability in solution under the conditions used for in vivo studies. The stability of 1 mg/mL solutions of domoic acid in saline, with or without ascorbic acid, was determined for 15 weeks. Solut...

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Bibliographic Details
Published inJournal of AOAC International Vol. 83; no. 2; p. 411
Main Author Johannessen, J N
Format Journal Article
LanguageEnglish
Published England 01.03.2000
Subjects
Ascorbic Acid
Chromatography, Liquid
Cold Temperature
Drug Stability
Kainic Acid - analogs & derivatives
Kainic Acid - analysis
Sodium Chloride
Solutions
Spectrophotometry, Ultraviolet
Temperature
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Summary:Studies designed to assess the effects of repeated low doses of domoic acid require an assessment of its stability in solution under the conditions used for in vivo studies. The stability of 1 mg/mL solutions of domoic acid in saline, with or without ascorbic acid, was determined for 15 weeks. Solutions were refrigerated, but warmed to room temperature for several hours each working day to simulate conditions of actual use. The solutions of domoic acid showed no evidence of decomposition as evidenced by stability of UV absorbance spectrum, concentration of domoic acid as determined by a liquid chromatographic method, and the chromatographic elution pattern. The addition of ascorbate to the domoic acid/saline solution did not alter the stability, but was deemed unnecessary because of the firm stability of the domoic acid/saline solution.
ISSN:1060-3271
DOI:10.1093/jaoac/83.2.411