Genome-Wide Analysis of Family-1 UDP-Glycosyltransferases in Potato ( Solanum tuberosum L.): Identification, Phylogenetic Analysis and Determination of Response to Osmotic Stress

• Published in: Genes Vol. 14; no. 12; p. 2144
• Main Authors: Wu, Yongchao, Liu, Jie, Jiao, Baozhen, Wang, Tingting, Sun, Sifan, Huang, Binquan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.11.2023
• Subjects: Abiotic stress; Amino acids; Flavonoids; Genes; Genome; Genomes; Glycosyltransferase; Glycosyltransferases - genetics; Mannitol; Molecular modelling; Osmotic Pressure; Osmotic stress; Phylogenetics; Phylogeny; Plant resistance; Potatoes; Proteins; Solanum tuberosum - genetics; Solanum tuberosum - metabolism; Transcriptomes; Transcriptomics; osmotic stress; expression pattern; potato; UDP-glycosyltransferase
• ISSN: 2073-4425; 2073-4425
• DOI: 10.3390/genes14122144

Family-1 UDP-glycosyltransferases (UGTs) are the most common and functional glycosyltransferases in the plant world. UGT is closely related to plant growth and the response to abiotic stress. However, despite systematic research, our understanding of potato UGT genes is still unclear. In this study, we identified 174 potato UGT proteins based on their conserved plant secondary product glycosyltransferase (PSPG) motifs. Phylogenetic analyses were used to compare these proteins with UGTs and other plant UGTs, and it was found that they could be clustered into 18 distinct groups. Patterns of intron gain/loss and intron phases within potato UGTs revealed highly conserved intron insertion events. The promoter -elements of these 174 genes were systematically investigated. The promoter regions of these genes are known to contain various classes of -acting compounds. These include elements that are light-responsive, phytohormone-responsive, and stress-responsive. Transcriptome data analysis established that 25, 10, 6, and 4 of these 174 genes were specifically expressed in leaves, roots, stolons, and young tubers, respectively. The mannitol-treated transcriptomic data showed thirty-eight genes were significantly upregulated. The quantitative real-time PCR results showed that the four genes were all responsive to osmotic stress under a 10% PEG6000 treatment. The results of our study provide a basis for clarifying the molecular mechanism of potato osmotic stress resistance and better understanding its function in the future.