Interleukin-8 gene expression by human dental pulp fibroblast in cultures stimulated with Prevotella intermedia lipopolysaccharide

• Published in: Journal of endodontics Vol. 22; no. 1; pp. 9 - 12
• Main Authors: Nagaoka, Shigetaka, Tokuda, Masayuki, Sakuta, Tetsuya, Taketoshi, Yuko, Tamura, Masato, Takada, Haruhiko, Kawagoe, Masataka
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 1996
• Subjects: Adolescent; Blotting, Northern; Cells, Cultured; Cytokines - pharmacology; Dental Pulp - cytology; Dental Pulp - immunology; Dental Pulp - metabolism; Dentistry; Dose-Response Relationship, Drug; Fibroblasts - drug effects; Fibroblasts - immunology; Fibroblasts - metabolism; Gene Expression; Humans; Interleukin-8 - biosynthesis; Lipid A - pharmacology; Lipopolysaccharides - immunology; Lipopolysaccharides - pharmacology; Male; Prevotella intermedia - immunology; RNA, Messenger - analysis; Salmonella - immunology
• ISSN: 0099-2399; 1878-3554
• DOI: 10.1016/S0099-2399(96)80228-7

Interleukin (IL)-8 mRNA expression was investigated in human dental pulp fibroblast cultures after stimulation with lipopolysaccharide (LPS) prepared from Prevotella intermedia and inflammatory cytokines. The expression of IL-8 mRNA and the release of IL-8 induced by P. intermedia LPS in pulpal fibroblast cultures were detected by Northern blot analysis and ELISA, respectively. The sufficient concentration of P. intermedia LPS on the IL-8 mRNA expression was 0.1 μg/ml in pulpal fibroblast cultures. IL-8 mRNA levels began to increase after 2 h of exposure, reached a maximum at 4 to 8 h, and declined after 48 h, reaching the unstimulated level by 60 h. IL-8 production by the pulpal fibroblasts began to increase after 8 h of exposure upon stimulation with 10 μg/ml of P. intermedia LPS. By contrast Salmonella LPS and synthetic lipid A did not increase IL-8 mRNA concentrations in pulpal fibroblast cultures. Recombinant human IL-1α, β, and tumor necrosis factor-α were capable of stimulating these cells to express IL-8 mRNA, but natural human interferon-β, γ, and recombinant human IL-6 were incapable in our assay. These results suggest that pulpal fibroblasts are immunoresponsive cells and can elaborate IL-8 upon stimulation with P. intermedia LPS.