Maliibacterium massiliense gen. nov. sp. nov., Isolated from Human Feces and Proposal of Maliibacteriaceae fam. nov

• Published in: Current microbiology Vol. 80; no. 6; p. 211
• Main Authors: Traore, Sory Ibrahima, Lo, Cheikh Ibrahima, Mossaab, Maaloum, Durand, Guillaume, Lagier, Jean Christophe, Raoult, Didier, Fournier, Pierre-Edouard, Fenollar, Florence
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2023; Springer Nature B.V; Springer Verlag
• Subjects: Adult; Bacteria; Bacterial Typing Techniques; Bacteriology; Biomedical and Life Sciences; Biotechnology; Cardiology and cardiovascular system; DNA, Bacterial - genetics; Emerging diseases; Fatty Acids; Feces; Feces - microbiology; Genes; Genomes; Genomics; Human health and pathology; Humans; Infectious diseases; Ions; Life Sciences; Male; Maximum likelihood method; Microbiology; Microbiology and Parasitology; New genera; New species; Nomenclature; Parasitology; Phylogenetics; Phylogeny; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Sheep; Short Communication; Software; Virology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-023-03301-4

Bacterial strain Marseille-P3954 was isolated from a stool sample of a 35-year-old male patient living in France. It was a gram-positive, rod-shaped anaerobic, non-motile, and non-spore-forming bacterium. C 16:0 and C 18:1n9 were the major fatty acid, while its genome measured 2,422,126 bp with 60.8 mol% of G+C content. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain Marseille-P3954 had 85.51% of similarity with Christensenella minuta , its closest related species with standing in nomenclature. As this value is very low compared to the recommended threshold, it suggested that the Marseille-P3954 strain belongs to a new bacterial genus, classified in a new family. On the basis of these genomic, phenotypic, and phylogenetic evidences, we propose that strain Marseille-P3954 should be classified as a new genus and species, Maliibacterium massiliense gen. nov., sp. nov. The type strain of M. massiliense sp. nov. is Marseille-P3954 (CSUR P3954 = CECT 9568).