Molecular characterization of 93 genotypes of cocoa (Theobroma cacao L.) with random amplified microsatellites RAMs

• Published in: Agronomía colombiana Vol. 32; no. 3; pp. 315 - 325
• Main Authors: Morillo C., Yacenia, Morillo C., Ana Cruz, Muñoz F., Jaime Eduardo, Ballesteros P., William, González, Alonso
• Format: Journal Article
• Language: English
• Published: Bogota Universidad Nacional de Colombia 01.12.2014; Universidad Nacional de Colombia, Facultad de Agronomía; Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
• Subjects: AGRONOMY; Cocoa; diversidad genética; Genotype & phenotype; heterocigosidad; Malvaceae; marcadores moleculares; Molecules; multiplicación clonal; Malvaceae; diversidad genética; heterozygosity; genetic diversity; heterocigosidad; marcadores moleculares; multiplicación clonal; molecular markers; clonal multiplication
• ISSN: 0120-9965; 2357-3732
• DOI: 10.15446/agron.colomb.v32n3.46879

Random amplified microsatellite (RAMs) markers six were used to characterize 93 genotypes of cocoa in Tumaco (Colombia). Hundred twenty seven bands were generated. The number of polymorphic loci varied between 11 and 25 for the AG and TG primers, respectively. This study differentiated the 93 genotypes into six groups with a 0.53 similarity, 0.28 mean heterozygosity (He) for the population, and 0.12±0.02 genetic differentiation coefficient or Fst. A significant level of genetic diversity was evident in the T. cacao genotypes. This resource would benefit selection programs of individual trees or plant breeding programs. The genotypes clustered in a large proportion in accordance with the collection zone. This characteristic was associated with collection zones and along the rivers in the municipality of Tumaco. The RAM technique proved to be a useful tool for the determination of genetic diversity in Theobroma species.