Cationic chitosan‐modified silica nanoparticles for oral delivery of protein vaccine

• Published in: Journal of biomedical materials research. Part A Vol. 109; no. 11; pp. 2111 - 2119
• Main Authors: Wu, Xi, Farooq, Muhammad Asim, Li, Tiantian, Geng, Tianjiao, Kutoka, Perpeuta Takunda, Wang, Bo
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.11.2021; Wiley Subscription Services, Inc
• Subjects: Administration, Oral; Animals; Antigens; Bovine serum albumin; Cations; Chitosan; Chitosan - chemistry; Chitosan - pharmacology; Coatings; Drug Delivery Systems; Electrophoresis; Female; Fourier analysis; Fourier transforms; Gel electrophoresis; Immune response; Infrared spectroscopy; mesoporous silica; Mice; Mice, Inbred BALB C; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - therapeutic use; oral delivery; Physiochemistry; Polyacrylamide; Proteins; Serum albumin; Serum Albumin, Bovine - chemistry; Serum Albumin, Bovine - pharmacology; Silica; Silicon dioxide; Silicon Dioxide - chemistry; Silicon Dioxide - pharmacology; Sodium dodecyl sulfate; Sodium lauryl sulfate; Structural stability; Surface charge; Transmission electron microscopy; Vaccines; Vaccines - chemistry; Vaccines - pharmacology; chitosan; mesoporous silica; bovine serum albumin; oral delivery
• ISSN: 1549-3296; 1552-4965; 1552-4965
• DOI: 10.1002/jbm.a.37198

Mesoporous silica nanoparticles coated with Chitosan are exploited here as a potential carrier for oral vaccine delivery. Bovine serum albumin (BSA) was used as a protein antigen model to reveal the carrier property. Chitosan‐coated BSA‐loaded silica NPs had particle size 345 ± 60 nm with a cationic surface charge of 18.28 ± 0.71 mV. The encapsulation efficiency, drug loading was 25.34 ± 0.76 and 20.21 ± 0.48%, respectively. Transmission electron microscopy investigation showed the spherical shape of NPs, also confirmed surface coating around modified nanoparticles (NPs), and nitrogen absorption/desorption isotherm confirmed mesostructured inside the NPs. Fourier transform infrared spectroscopy did not show any physiochemical interactions between excipients and formulations. The structural stability of antigen after release from NPs was confirmed by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis analysis, and chitosan‐coated silica NPs exhibited a slow‐release pattern. The results of in vivo experiments presented that chitosan‐mesoporous silica NPs could induce a robust immune response in mice, indicating that chitosan‐mesoporous silica NPs might be used as a promising carrier for oral vaccine delivery.