Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 108; no. 4; pp. 1697 - 1709
• Main Authors: Ressler, Antonia, Cvetnić, Matija, Antunović, Maja, Marijanović, Inga, Ivanković, Marica, Ivanković, Hrvoje
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.05.2020; Wiley Subscription Services, Inc
• Subjects: Biomedical materials; Biomimetics; Body fluids; Calcite; Calcium; Calcium carbonate; Calcium phosphates; Carbonation; Cell culture; Cell proliferation; Cell viability; cosubstitution; Culture; Fourier analysis; Fourier transforms; Heat treatment; Heat treatments; Hydroxyapatite; In vitro methods and tests; Infrared analysis; Lattice parameters; Magnesium; Materials research; Materials science; Octacalcium phosphate; Phase transitions; Reagents; Rietveld refinement; Spectrometry; Strontium; Substitutes; Surgical implants; Tricalcium phosphate; hydroxyapatite; Rietveld refinement; cosubstitution; octacalcium phosphate; strontium
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.34515

Biomimetic triphasic strontium‐substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO3, (NH2)2COH3PO4, and Sr(NO3)2 as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X‐ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β‐tricalcium phosphate (β‐TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β‐TCP. Sr2+ occupy the Ca(1) site in HA, Ca(3,4,7,8) sites in OCP and Ca(1,2,3,4) sites in β‐TCP. Along with Sr2+ substitution, presence of Mg2+ and Na+ ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture.