Prognostic significance of Ki67 evaluated by flow cytometry in dogs with high‐grade B‐cell lymphoma

Ki67 can discriminate between high‐ and low‐grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67‐positive cells), evaluated by flow cytometry, in 40 dogs with high‐grade B‐cell lymphoma,...

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Published inVeterinary & comparative oncology Vol. 15; no. 2; pp. 431 - 440
Main Authors Poggi, A., Miniscalco, B., Morello, E., Gattino, F., Delaude, A., Ferrero Poschetto, L., Aresu, L., Gelain, M. E., Martini, V., Comazzi, S., Riondato, F.
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.06.2017
Subjects
Animals
dog
Dog Diseases - blood
Dog Diseases - diagnosis
Dog Diseases - drug therapy
Dog Diseases - mortality
Dogs
Female
flow cytometry
Flow Cytometry - methods
Flow Cytometry - veterinary
Ki-67 Antigen - blood
Ki67
lymphoma
Lymphoma, B-Cell - blood
Lymphoma, B-Cell - diagnosis
Lymphoma, B-Cell - drug therapy
Lymphoma, B-Cell - veterinary
Male
Prognosis
Survival Analysis
flow cytometry
Ki67
dog
prognosis
lymphoma
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Summary:Ki67 can discriminate between high‐ and low‐grade canine lymphomas, but its prognostic role in specific subtypes of the neoplasm is unknown. We assessed the prognostic significance of Ki67% (percentage of Ki67‐positive cells), evaluated by flow cytometry, in 40 dogs with high‐grade B‐cell lymphoma, treated with a modified Wisconsin–Madison protocol (UW‐25). The following variables were investigated for association with lymphoma specific survival (LSS) and relapse free interval (RFI): Ki67%, breed, sex, age, stage, substage, complete remission (CR). By multivariate analysis, Ki67% (P = 0.009) and achievement of CR (P = 0.001) were independent prognostic factors for LSS. Dogs with intermediate Ki67% (20.1–40%) presented longer LSS and RFI (median = 866 and 428 days, respectively) than dogs with low (median = 42 days, P < 0.001; median = 159 days, P = 0.014) or high (median = 173 days, P = 0.038; median = 100 days, P = 0.126) values. Determination of Ki67 is a prognostic tool that improves the clinical usefulness of flow cytometric analysis in canine high‐grade B‐cell lymphoma.
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ISSN:1476-5810
1476-5829
DOI:10.1111/vco.12184