Methomyl Detection by Inhibition of Laccase Using a Carbon Ceramic Biosensor

• Published in: Electroanalysis (New York, N.Y.) Vol. 23; no. 7; pp. 1623 - 1630
• Main Authors: Cadorin Fernandes, Suellen, Cruz Vieira, Iolanda, Barbosa, Antonio Marcos Jacques, Souza Ferreira, Valdir
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.07.2011; WILEY‐VCH Verlag
• Subjects: Biosensors; Carbon; Ceramics; Confidence intervals; Esculetin; Inhibition; Laccase; Mathematical analysis; Methomyl; Sol-gel; Vegetables
• ISSN: 1040-0397; 1521-4109
• DOI: 10.1002/elan.201100044

A sol–gel derived carbon ceramic biosensor was used for methomyl determination in vegetable extract samples based on the immobilization of laccase from Aspergillus oryzae. Esculetin was chosen as the substrate for laccase in order to measure inhibition by this pesticide. The analytical curve was linear for methomyl concentrations of 0.5 to 12.2 µM with a detection limit of 0.2 µM. The lifetime of the proposed biosensor was 60 days and the recovery from vegetable extract samples ranged from 98.0 to 104.2 %. The results using the proposed method are in agreement with those using HPLC at the 95 % confidence level.