In vitro evaluation of loaded chitosan membranes for pain relief and infection prevention

• Published in: Journal of biomedical materials research. Part B, Applied biomaterials Vol. 109; no. 11; pp. 1735 - 1743
• Main Authors: Harrison, Zoe L., Bumgardner, Joel D., Fujiwara, Tomoko, Baker, Daniel L., Jennings, J. Amber
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.11.2021; Wiley Subscription Services, Inc
• Subjects: anesthetic; Anesthetics; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Bacteria; Bandages; biofilm; Biofilms; biomaterial; Biomedical materials; Bupivacaine; Chitosan; Chitosan - chemistry; Drug Evaluation, Preclinical; Drug resistance; electrospun; Fibroblasts; Hexanoic acid; infection; Infections; Local anesthetics; local drug delivery; Materials research; Materials science; Medical equipment; Membranes; Membranes, Artificial; Methicillin; Methicillin-Resistant Staphylococcus aureus - growth & development; Minimum inhibitory concentration; Pain; Pain - drug therapy; Staphylococcal Infections - drug therapy; staphylococcus; Staphylococcus aureus; Strains (organisms); Trauma; wound dressing; chitosan; anesthetic; infection; wound dressing; biomaterial; bupivacaine; electrospun; biofilm; staphylococcus; local drug delivery
• ISSN: 1552-4973; 1552-4981
• DOI: 10.1002/jbm.b.34831

Wounds resulting from surgeries, implantation of medical devices, and musculoskeletal trauma result in pain and can also result in infection of damaged tissue. Up to 80% of these infections are due to biofilm formation either on the surface of implanted devices or on surrounding wounded tissue. Bacteria within a biofilm have intrinsic growth and development characteristics that allow them to withstand up to 1,000 times the minimum inhibitory concentration of antibiotics, demonstrating the need for new therapeutics to prevent and treat these infections. Cis‐2‐decenoic acid (C2DA) is known to disperse preformed biofilms and can prevent biofilm formation entirely for some strains of bacteria. Additionally, local anesthetics like bupivacaine have been shown to have antimicrobial effects against multiple bacterial strains. This study sought to evaluate hexanoic acid‐treated electrospun chitosan membranes (HA‐ESCM) as wound dressings that release C2DA and bupivacaine to simultaneously prevent infection and alleviate pain associated with musculoskeletal trauma. Release profiles of both therapeutics were evaluated, and membranes were tested in vitro against Methicillin‐resistant Staphylococcus aureus (MRSA) to determine efficacy in preventing biofilm infection and bacterial growth. Results indicate that membranes release both therapeutics for 72 hr, and release profile can be tailored by loading concentration. Membranes were effective in preventing biofilm growth but were toxic to fibroblasts when loaded with 2.5 or 5 mg of bupivacaine.